| Disposal Of Used Media/Microbial Culture And Dehydrated Media |
1.0 OBJECTIVE To lay down a Procedure for the disposal of used media/microbial cultures and dehydrated media. 2.0 SCOPE This procedure is applicable for disposal of used media/microbial cultures and dehydrated media in the Quality Control Department of Microbiology Area. 3.0 RESPONSIBILITY • Lab-attend/Microbiologist: is responsible for carrying out the established procedure • Head of Microbiology : is responsible for ensuring that the SOP is followed correctly. 4.0 ACCOUNTABILITY • Department Head • Head Quality / QA To Read this SOP click here SOP for Cleaning of waste-bin and drain in microbiology department. 5.0 PROCEDURE 5.1 Safety consideration 5.1.1 The material should be transported in closed stainless steel containers, from the place of use to the place where they are decontaminated and disposed off. 5.1.2 Use gloves, nose mask, apron and goggles during handling of disposable material. 5.1.3 Disinfect hands after completion of work. 5.1.4 Discarding shall be done by trained person. 5.1.5 Requirements 5.1.6 Yellow discard polybags 5.1.7 Surgical gloves 5.1.8 Vertical autoclave 5.1.9 Disinfectant 5.1.10 Black polybags 5.1.11 Container 5.2 Disposal of used media/Microbial Culture: 5.2.1 Transfer the use media petri plates, media test tubes and microbial cultures tubes to be disposed in washing area of the laboratory. 5.2.2 Collect all used media petri plates, media test tubes in Yellow discard polybag and properly wrap the polybag. 5.3 Disposal of Dehydrated Media: 5.3.1 Transfer the quantity of dehydrated media in discarded yellow polybag and reconstitute the quantity with water in the ratio 1/10 (1gm of media reconstituted with 9mL water) and add 500ml sanitizing agent (as per schedule). 5.4 Discard cycle 5.4.1 Tie the polybag tightly and Affix a chemical indicator tape on each poly bags and one chemical indicator tape any one poly bags. 5.4.2 Keep the polybags on basket of vertical autoclave at start the autoclave as per respective Sop. Run cycle at 121.1°c for 30minutes 5.4.3 After completion of the discard cycle, open the lid of autoclave take out the polybag disposable bag. 5.4.4 Record the load details in autoclave usage log record and paste the chemical indicator in respective format of each load. 5.4.5 Open the disposable bag to allow the contents (media plates, test tubes etc.) to cool. 5.4.6 After cooling, remove all the media from the petri plates with the help of a spatula and collect into a another yellow polybag having 500ml disinfectant agents as per schedule. 5.4.7 Tie the poly bag tightly with cable tie and paste a label it as “Decontaminated Media/Waste for final disposal” put signature and date on the label. Decontaminated Media/Waste for final disposal Signature : Date : 5.4.8 Now carry polybag securely in a container to ETP kept in dedicated decontaminated media container. 5.4.9 Admin/microbiologist/engineering person shall check the decontaminated media send to third party for discompose of media. 5.4.10 Decontaminated media destruction, run an empty cycle at 121°c for 30 minutes. 5.5 Cleaning of glassware 5.5.1 Immerse the used empty petri plates, tubes, and other glassware dip in water for injection for about 15minutes. 5.5.2 Do further washing. Cleaning and drying of then as per current Sop No. QC04-019 “Cleaning of the glassware in microbiology laboratory. 5.6 Inspect the chemical indicator in each cycle of media destruction and empty cycle and observe colour change of indicator. If indicator colour does not change then run the cycle again with new chemical indicator, and investigate the reason for failure of discard cycle or empty cycle. 5.7 Verification of discard media: 5.7.1 Make any one plates/tubes which have microbial growth or pure microbial culture and keep it in discard cycle of autoclave along with other media. 5.7.2 After media cycle of autoclave take marked plates/tubes and transfer in microbial testing area though pass box. 5.7.3 Take 02 to 05ml aliquot plates/tubes and aseptically pour in a 100ml SCDM media tube. 5.7.4 After transferring aliquot in the tube containing 100mL SCDM, incubate this tube for 48hrs. at 30-35°c. 5.7.5 Record the verification detail in format no. XXX. 5.7.6 After incubation growth should not observed in the SCDM tube, in case is growth observed then check the load configuration and inform to department head for rectification. |
