| Operation and Calibration of UV Cabinet |
1.0 PURPOSE 1.1 To Define procedure for operation and calibration of UV cabinet in quality control department. 2.0 SCOPE 2.1 This procedure for operation and calibration of UV cabinet in quality control department. 3.0 REFERENCE(S) & ATTACHMENTS 3.1 In house 3.2 Attachments 3.3 Attachment –I : Calibration of UV Cabinet 4.0 RESPONSIBILITY 4.1 Quality control Officer/ Executive: 4.1.1 To initiate the preparation of SOP through the inputs from the employees directly concerned with the activity in quality control department. 4.1.2 To prepare the SOP and list of all assigned numbers of the SOP for their departments. 4.2 Quality Control Head: 4.2.1 To confirm the content, accuracy and detailing about of SOP. 4.2.2 To organize the approval of SOP from Quality assurance (QA head) and Location Head. 4.2.3 To ensure the training of the SOP to all concerned involved in implementation of the SOP. 4.2.4 To ensure user the implementation of the SOP after training. 4.3 Quality Assurance Department 4.3.1 To check the SOP and maintain the original copy of the SOP. 4.3.2 To issue controlled copy of the SOP and maintain issuance record of SOP. 4.3.3 To retrieve the controlled copy of the SOP when it is superseded/obsolete. 4.3.4 To control and monitor the review process of the SOP. 4.4 Quality Assurance Head & Location Head 4.4.1 To review and approve SOP. 4.4.2 To ensure the implementation of the defined system in quality control department. 5.0 DISTRIBUTION 5.1 Quality control department 6.0 ABBREVIATION(S) 6.1 SOP : Standard Operating Procedure 6.2 QA : Quality Assurance 6.3 QC : Quality Control 6.4 No. : Number 6.5 VAC : Voltage Alternate current 6.6 NA : Not Applicable 6.7 UV : Ultraviolet 6.8 TLC : Thin Layer Chromatography 7.0 PROCEDURE 7.1 Equipment Details As per written on instrument DQ. 7.2 Operation 7.2.1 Plug the power cord unit into V AC mains and switch on the mains. 7.2.2 Switch on the VISIBLE LIGHT / LONG UV (365nm) / SHORT UV (254 nm) to select the wavelength on which the material is to be measured. 7.2.3 Open the lid and place the TLC plate inside the cabinet. Close the lid and observe the spots. 7.2.4 Switch off the mains after use. 7.3 Maintenance 7.3.1 Wipe the outside surface of the instrument with Colin solution followed by a Fine duster. 7.3.2 Interior maintenance of the instrument should be done by service engineer whenever needed. 7.4 Calibration 7.4.1 Prepare 0.04 percent w/v solution of Sodium Salicylate in Ethanol (95%) and 0.2 percent w/v solution of Sodium Salicylate in Ethanol (95%). 7.4.2 Apply 5µl each of the above solution on the TLC plate run. 7.4.3 Leave it for drying after drying observe the TLC plate under the wave length of 254 nm & 365 nm. 7.4.4 Spots of the above solution should be clearly visible. measure the diameter of spots. It should be at about 5 mm. 7.5 Frequency of Calibration 7.5.1 Frequency of calibration is after three months and after each maintenance job. 7.6 Precautions 7.6.1 Do not directly see to UV light and do not expose hand in to UV light. |
