| Operation and Cleaning of Anaerobic Jar |
1.0 OBJECTIVE To lay down a Procedure for operation and cleaning of anaerobic jar. 2.0 SCOPE This procedure is applicable for cleaning and operation anaerobic jar in the Quality Control Department of Microbiology Area. 3.0 RESPONSIBILITY • Lab-attendant/Microbiologist: is responsible for carrying out the established procedure • Head of Microbiology : is responsible for ensuring that the SOP is followed correctly. 4.0 ACCOUNTABILITY • Department Head 5.0 PROCEDURE (operation and cleaning of anaerobic jar) 5.1 Principle: Anaerobic jars, stainless steel for the cultivation of anaerobic and microaerophilic microorganism in aa defined and rapidly generated gas atmosphere. The requested atmosphere may be reached under ideal condition by two methods. Either by using chemical gas packs (anaerobe system) or by manually evacuating the jars with a vacuum pump and flushing with gas afterwards (e.g. with nitrogen), in this case no chemical accessories are needed (expect anaerobic jar eco and crystal eco.) The jars are of robust stainless steel. The lids are made of stainless steel with two valves and include tube clips for vacuum hoses and with manometer for exact control of the vacuum or overpressure. The optional racks are made of stainless steel providing holders for comfortable operation of the anaerobe system. 5.2 Oxygen is removed dorm the jar by catalytic reaction. The catalyst activates hydrogen gas introduced into jars by activation of gas pack disposable hydrogen+ carbon dioxide generator envelops to combine with free oxygen in the jar to form water. To Read this SOP Click Here SOP for Cleaning Operation and Qualification of incubator. 5.3 operation and cleaning of anaerobic jar 5.3.1 Check and ensure that the anaerobic jar is clean and suitable for starting the operation, if not, clean the jars, if not, clean with a soft cloth duster. 5.3.2 Open the lid and place it carefully on the bench or suitable place with catalyst sachet uppermost, to reduce the risk of its contamination. 5.3.3 Load the culture into the jar on their dished or containers. 5.3.4 Replace the lid on the jar and secure it the bridge clamp, finger tightening the Bakelite knob. 5.3.5 Shut both needle valves. 5.3.6 Open the vacuum needle valve and allow the pressure in the jar to fall to or below 10cm mercury. The cover will tend to pull under vacuum. 5.3.7 Close the valve after adding the gas packs and remove both hydrogen and vacuum connection. 5.3.8 incubate the jar at 30-35°c for the required period. 5.3.9 After incubation, remove the bridge clamp and open one of the valves so that any reduced pressure inside the jar will be release and the covered can be removed. 5.3.10 The catalyst which is provided to catalyze the reaction between residual oxygen and added hydrogen normally remains active for long periods. 5.3.11 To activates the catalyst before a cycle, it should be heated in an oven for 90 minutes 160°c. 5.4 Incase using gas pack. 5.4.1 Check and ensure that the anaerobic jar is clean and suitable for starting the operation. 5.4.2 Open the clamp of anaerobic jar. 5.4.3 Place the petri plates/glassware to be incubated in the jar. 5.4.4 Remove the paper sachet from foil bag of the anaerogas pack, do not open the paper sachet. 5.4.5 Immediately place the paper sachet and add the anaero indicator tablet into the anaerobic jar and clamp the jar lid. 5.4.6 Incubate the jar at the required temperature for the required time period. 5.4.7 The pick colour of the indicator tablets indicated anaerobic condition. 5.4.8 After incubation clean the jar with 70% IPA. 5.4.9 Discard the sachet with care, don’t throw the sachet with combustibles. |