| 0.004 M Benzethonium Chloride |
| 1.0 Scope: This procedure is applicable for preparation and standardization of 0.004 M Benzethonium chloride solution. 2.0 Apparatus: 2.1 Auto titrator 2.2 1000 ml and 100 ml volumetric flask 2.3 250 ml glass-stoppered, conical flask 2.4 10 ml volumetric pipette 3.0 Reagents: 3.1 Benzethonium chloride(C27H42ClNOH2) AR grade 3.2 Sodium lauryl sulfate 3.3 Dilute sulphuric acid 3.4 Chloroform 3.5 dimethyl yellow-orcet blue-B- solution To read this GTP click here Preparation and Standardization of 0.05 M Bromine. 4.0 Preparation of Reagents: Dimethyl yellow-orcet blue-B- solution: Dissolve 15.0 mg of dimethyl yellow and 15.0 mg orcet blue-B- in chloroform and dilute to 500.0 ml with chloroform. 5.0 Preparation: Dissolve 1.792 g of benzethonium chloride, previously dried to constant weight at 105 degree C, in sufficient purified water to produce 1000 ml. 6.0 Standardization Weigh accurately about 570.0 mg of Sodium lauryl sulfate, previously dried at about 105 degree Celsius for about 1 hour. Transfer it to a cleaned and dried 500.0 ml volumetric flask and add 300.0 ml of water with gentle stirring. Make up the volume 500.0 ml with water. Mix solution thoroughly (Solution-A) Take 20.0ml of solution-A add 10 ml of water, 15.0 ml chloroform, 10.0 ml dilute sulphuric acid and add 1 ml dimethyl yellow-orcet Blue-B- solution as indicator. Titrate with Benzethonium chloride solution. 7.0 Calculation: W (in gm) X 20 N=——————————– Volume cons. X 0.28838 X 500 Were: N = molarity of 0.004 M C27H42ClNOH2 W = weight of C27H42ClNOH2 in gm. Volume Cons. =Consumed volume 0.004 M Benzethonium chloride in ml. 8.0 Storage: Store in a well fitted transparent glass bottle. |
