| Cleaning And Sterilization Of Glassware’s in Microbiology Laboratory |
1.0 OBJECTIVE To lay down a Procedure for Procedure for cleaning and sterilization of glassware’s in microbiology laboratory. 2.0 SCOPE This procedure is applicable for Procedure for cleaning and sterilization of glassware’s in the Quality Control Department of Microbiology Area. 3.0 RESPONSIBILITY • Lab-attendant/Microbiologist : is responsible for cleaning and sterilization of glassware as per SOP. • Manager microbiology : is responsible for ensuring that the SOP is followed correctly 4.0 ACCOUNTABILITY • Department Head • Head Quality / QA 5.0 PROCEDURE 5.1 Safety considerations: 5.1.1 While handling used Petri plate, pipettes, flask and bottle wear disposable hand gloves and mask. 5.1.2 All contaminated glassware (Petri plates, test tubes and media flask etc.) before cleaning discard as per current receptive SOP “Disposal of used media/microbial culture and dehydrated media. 5.1.3 Always take care of breakage during cleaning. 5.1.4 Glass piece must be removed immediately in case of breakage. 5.1.5 Cleaning agents may be hazardous and may be carefully. To read this sop click here SOP for Media preparation Consumption and Sterilization in Microbiology Laboratory. 5.2 Cleaning of glassware’s 5.2.1 Petri plates/media test tubes and culture tubes 5.2.1.1 Remove the marking on the plates and tubes with70% IPA. 5.2.1.2 Dispose all the plates and cultures tubes as per respective Sop. 5.2.1.3 After the destruction cycle is over, remove the agar from the plates and collect the discarded agar media in Yellow colour bio-hazards autoclavable bags. 5.2.1.4 Dip the plates and test tubes in a wash tub with 2.5% DettolSolution for about 01 hours. 5.2.1.5 After one hour, rinse the plates and cultured tubes with tap water and again dip then in wash tab/bucket. 5.2.1.6 Scrub each plate with soft scrubber 5.2.1.7 Wash each plate thoroughly with soft water to removed soap solution. 5.2.1.8 Finally rinse with purified water. 5.2.1.9 Keep the plates for drying in oven at 70-80°c. 5.2.1.10 After drying pack 10-12 Petri plates in bundle and warp with butter paper/bacteriological paper. 5.2.1.11 Prior to use, sterilize the article required for aseptic work. 5.2.2 For manifold unites 5.2.3.1 Disassemble the unit and wash thoroughly with 2.5% Teepol solution/soap solution to gasket, lid and interior and exterior surface of units. 5.2.3.2 Wash the unit with tap water. 5.2.3.3 Finally rinse the unit purified water and keep for drying. 5.2.3.4 Assemble the unit for prior to use for sterilization. 5.2.3 For volumetric flask, beaker, conical flask, test tubes and screw cap bottles. 5.2.3.1 Bring all used glassware to the washing area. 5.2.3.2 Remove any marking on the glassware’s with 70% IPA. 5.2.3.3 Drain out the solution from glassware completely and wash these with tap water to remove the solution, scrub the interior & exterior surface of the glassware’s with 2.5% Teepol solution/soap solution using a long handle nylon brush. 5.2.3.4 Drain out the soap solution and wash the glassware thoroughly with soft water to remove the soap solution completely and the finally rinse with purified water. 5.2.3.5 Dry the glassware in hot air oven at 70-80°c. 5.2.4 Test tubes bacterial Endotoxin Test 5.2.4.1 The contents in the BET assay tubes shall be discarded in the glass baker. 5.2.4.2 The assay tubes shall be cleaned only with plenty water for injection and no cleaning agents or for soft water shall be used. 5.2.4.3 The washed glassware shall be placed in to hot air oven for drying at 70-80°C. 5.2.4.4 After drying the BET test tubes shall be packed for depyrogenation cycle at more than 250°c dry heat sterilization 5.2.5 Sterilization of glassware’s 5.2.5.1 Before sterilization ensures that the autoclave should be clean and dry and check the all utility. 5.2.5.2 All the petri plates shall be wrapped in a layer of butter paper/bacteriological paper and place in Place in autoclave. 5.2.5.3 Screw cap sampling bottles shall be loosely capped and wrapped with butter paper/bacteriological paper. 5.2.5.4 Filtration flask, cups, forceps also wrapped with butter paper/bacteriological paper. 5.2.5.5 Test tubes are plugged with cotton plug or close by screw cap when sterilized in autoclave. 5.2.5.6 Load there wrapped glassware in an autoclave. 5.2.5.7 Affix a label if require having date of sterilization. 5.2.5.8 Operate the autoclave as per respective sop and sterilized the material by autoclaving at 121.1 to 123.9°c as per validated cycle time. 5.2.5.9 After the completion of sterilization cycle, autoclave is opened, the screw capped bottles are screwed tightly and the material from the autoclave in unloaded. 5.2.5.10 Chemical indicator shall be placed on every material to verify the sterilization cycle. |
