1.0 what is the ultraviolet and ultraviolet visible range in UV- Vis Spectrophotometer?
Answer:- The Uv range in UV-Vis Spectrophotometer is 200nm to 400nm and uv visible range is 400nm to 800nm.
To read this SOP click here SOP for Operation of Infrared Moisture Balance.
2.0 what is the calibration parameter of UV-Vis Spectrophotometer?
Answer. These are parameter used in UV-Vis Spectrophotometer calibration
• Control of Wavelength
• Control of Absorbance
• Limit of Stray light
• Resolution power
• Second Order Derivative
• Solvents
• Cell Transmittance
3.0 How to Prepared of 0.005 M sulphuric acid for calibration parameter of Control of Absorbance?
Answer: Add 0.6 ml of concentrated sulphuric acid (AR grade) in 2000 ml of purified water.
4.0 How to performed to Solvents parameter?
Answer: Take the absorbance of following solvents at about 254 nm with reference to water.
• Ethanol (95%)
• Ethanol
• Methanol
• Cyclohexane
Limit: The absorbance should not more than 0.10
5.0 what is the full form of nm in uv spectrophotometer?
Answer: nm full form is nanometre in uv-vis spectrophotometer.
6.0 what is beer lambert law for uv-vis spectrophotometer?
Answer: Beer lambert law say that if increase the concentration of any solution so that absorbance will be increase. concentration is directly proportional to absorbance.